Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis

Delia Bucher, Felix Frey, Kem A. Sochacki, Susann Kummer, Jan-Philip Bergeest, William J. Godinez, Hans-Georg Kräusslich, Karl Rohr, Justin W. Taraska, Ulrich S. Schwarz () and Steeve Boulant ()
Additional contact information
Delia Bucher: University Hospital Heidelberg
Felix Frey: BioQuant Center
Kem A. Sochacki: National Institutes of Health
Susann Kummer: University Hospital Heidelberg
Jan-Philip Bergeest: German Cancer Research Center (DKFZ)
William J. Godinez: German Cancer Research Center (DKFZ)
Hans-Georg Kräusslich: University Hospital Heidelberg
Karl Rohr: German Cancer Research Center (DKFZ)
Justin W. Taraska: National Institutes of Health
Ulrich S. Schwarz: BioQuant Center
Steeve Boulant: University Hospital Heidelberg

Nature Communications, 2018, vol. 9, issue 1, 1-13

Abstract: Abstract Although essential for many cellular processes, the sequence of structural and molecular events during clathrin-mediated endocytosis remains elusive. While it was long believed that clathrin-coated pits grow with a constant curvature, it was recently suggested that clathrin first assembles to form flat structures that then bend while maintaining a constant surface area. Here, we combine correlative electron and light microscopy and mathematical growth laws to study the ultrastructural rearrangements of the clathrin coat during endocytosis in BSC-1 mammalian cells. We confirm that clathrin coats initially grow flat and demonstrate that curvature begins when around 70% of the final clathrin content is acquired. We find that this transition is marked by a change in the clathrin to clathrin-adaptor protein AP2 ratio and that membrane tension suppresses this transition. Our results support the notion that BSC-1 mammalian cells dynamically regulate the flat-to-curved transition in clathrin-mediated endocytosis by both biochemical and mechanical factors.

Date: 2018
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-018-03533-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03533-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-03533-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().