O2 evolution and recovery of the water-oxidizing enzyme

Keisuke Kawashima, Tomohiro Takaoka, Hiroki Kimura, Keisuke Saito and Hiroshi Ishikita ()
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Keisuke Kawashima: The University of Tokyo, 7-3-1 Hongo
Tomohiro Takaoka: The University of Tokyo, 7-3-1 Hongo
Hiroki Kimura: The University of Tokyo, 7-3-1 Hongo
Keisuke Saito: The University of Tokyo, 7-3-1 Hongo
Hiroshi Ishikita: The University of Tokyo, 7-3-1 Hongo

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract In photosystem II, light-induced water oxidation occurs at the Mn4CaO5 cluster. Here we demonstrate proton releases, dioxygen formation, and substrate water incorporation in response to Mn4CaO5 oxidation in the protein environment, using a quantum mechanical/molecular mechanical approach and molecular dynamics simulations. In S2, H2O at the W1 site forms a low-barrier H-bond with D1-Asp61. In the S2-to-S3 transition, oxidation of OW1H– to OW1•–, concerted proton transfer from OW1H– to D1-Asp61, and binding of a water molecule Wn-W1 at OW1•– are observed. In S4, W n -W1 facilitates oxo-oxyl radical coupling between OW1•– and corner μ-oxo O4. Deprotonation via D1-Asp61 leads to formation of OW1=O4. As OW1=O4 moves away from Mn, H2O at W539 is incorporated into the vacant O4 site of the O2-evolved Mn4CaO4 cluster, forming a μ-oxo bridge (Mn3–OW539–Mn4) in an exergonic process. Simultaneously, Wn-W1 is incorporated as W1, recovering the Mn4CaO5 cluster.

Date: 2018
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DOI: 10.1038/s41467-018-03545-w

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