AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling

Julian Dindas, Sönke Scherzer, M. Rob G. Roelfsema, Katharina Meyer, Heike M. Müller, K. A. S. Al-Rasheid, Klaus Palme, Petra Dietrich, Dirk Becker, Malcolm J. Bennett and Rainer Hedrich ()
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Julian Dindas: University of Würzburg
Sönke Scherzer: University of Würzburg
M. Rob G. Roelfsema: University of Würzburg
Katharina Meyer: University of Würzburg
Heike M. Müller: University of Würzburg
K. A. S. Al-Rasheid: King Saud University
Klaus Palme: Centre for Biological Systems Analysis
Petra Dietrich: University of Erlangen-Nürnberg
Dirk Becker: University of Würzburg
Malcolm J. Bennett: University of Nottingham
Rainer Hedrich: University of Würzburg

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration- and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA- triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots.

Date: 2018
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DOI: 10.1038/s41467-018-03582-5

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