De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis

Chunming Ding (), Yen Ching Lim, Sook Yoong Chia, Arcinas Camille Esther Walet, Shaohai Xu, Kinyui Alice Lo, Yanling Zhao, Dewen Zhu, Zhihui Shan, Qingfeng Chen, Melvin Khee-Shing Leow, Dan Xu () and Lei Sun ()
Additional contact information
Chunming Ding: Wenzhou Medical University
Yen Ching Lim: Wenzhou Medical University
Sook Yoong Chia: Duke-NUS Medical School
Arcinas Camille Esther Walet: Duke-NUS Medical School
Shaohai Xu: Nanyang Technological University
Kinyui Alice Lo: Institute of Molecular and Cell Biology
Yanling Zhao: Wenzhou Medical University
Dewen Zhu: Wenzhou Medical University
Zhihui Shan: Wenzhou Medical University
Qingfeng Chen: Institute of Molecular and Cell Biology
Melvin Khee-Shing Leow: Duke-NUS Medical School
Dan Xu: Wenzhou Medical University
Lei Sun: Duke-NUS Medical School

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.

Date: 2018
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-018-03754-3 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03754-3

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-03754-3

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().