Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo

Richard T. O’Neil, Sunandan Saha, Ruth Ann Veach, Richard C. Welch, Lauren E. Woodard, Cliona M. Rooney and Matthew H. Wilson ()
Additional contact information
Richard T. O’Neil: Vanderbilt University School of Medicine
Sunandan Saha: Baylor College of Medicine
Ruth Ann Veach: Vanderbilt University School of Medicine
Richard C. Welch: Vanderbilt University School of Medicine
Lauren E. Woodard: Vanderbilt University School of Medicine
Cliona M. Rooney: Center for Cell and Gene Therapy, Baylor College of Medicine
Matthew H. Wilson: Vanderbilt University School of Medicine

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract A cell therapy platform permitting long-term delivery of peptide hormones in vivo would be a significant advance for patients with hormonal deficiencies. Here we report the utility of antigen-specific T lymphocytes as a regulatable peptide delivery platform for in vivo therapy. piggyBac transposon modification of murine cells with luciferase allows us to visualize T cells after adoptive transfer. Vaccination stimulates long-term T-cell engraftment, persistence, and transgene expression enabling detection of modified cells up to 300 days after adoptive transfer. We demonstrate adoptive transfer of antigen-specific T cells expressing erythropoietin (EPO) elevating the hematocrit in mice for more than 20 weeks. We extend our observations to human T cells demonstrating inducible EPO production from Epstein–Barr virus (EBV) antigen-specific T lymphocytes. Our results reveal antigen-specific T lymphocytes to be an effective delivery platform for therapeutic molecules such as EPO in vivo, with important implications for other diseases that require peptide therapy.

Date: 2018
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-018-03787-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03787-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-03787-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().