Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

Megumi Shiota, Masayuki Naya (), Takehiro Yamamoto, Takako Hishiki, Takeharu Tani, Hiroyuki Takahashi, Akiko Kubo, Daisuke Koike, Mai Itoh, Mitsuyo Ohmura, Yasuaki Kabe, Yuki Sugiura, Nobuyoshi Hiraoka, Takayuki Morikawa, Keiyo Takubo, Kentaro Suina, Hideaki Nagashima, Oltea Sampetrean, Osamu Nagano, Hideyuki Saya, Shogo Yamazoe, Hiroyuki Watanabe and Makoto Suematsu ()
Additional contact information
Megumi Shiota: FUJIFILM Corporation
Masayuki Naya: FUJIFILM Corporation
Takehiro Yamamoto: Keio University School of Medicine
Takako Hishiki: Keio University School of Medicine
Takeharu Tani: FUJIFILM Corporation
Hiroyuki Takahashi: FUJIFILM Corporation
Akiko Kubo: Keio University School of Medicine
Daisuke Koike: FUJIFILM Business Expert Corporation
Mai Itoh: Keio University School of Medicine
Mitsuyo Ohmura: Keio University School of Medicine
Yasuaki Kabe: Keio University School of Medicine
Yuki Sugiura: Keio University School of Medicine
Nobuyoshi Hiraoka: National Cancer Center Research Institute
Takayuki Morikawa: National Center for Global Health and Medicine
Keiyo Takubo: National Center for Global Health and Medicine
Kentaro Suina: Keio University School of Medicine
Hideaki Nagashima: Keio University School of Medicine
Oltea Sampetrean: Keio University School of Medicine
Osamu Nagano: Keio University School of Medicine
Hideyuki Saya: Keio University School of Medicine
Shogo Yamazoe: FUJIFILM Corporation
Hiroyuki Watanabe: FUJIFILM Corporation
Makoto Suematsu: Keio University School of Medicine

Nature Communications, 2018, vol. 9, issue 1, 1-16

Abstract: Abstract Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.

Date: 2018
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DOI: 10.1038/s41467-018-03899-1

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