Self-replication of DNA by its encoded proteins in liposome-based synthetic cells

Pauline van Nies, Ilja Westerlaken, Duco Blanken, Margarita Salas, Mario Mencía and Christophe Danelon ()
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Pauline van Nies: Delft University of Technology
Ilja Westerlaken: Delft University of Technology
Duco Blanken: Delft University of Technology
Margarita Salas: Universidad Autónoma
Mario Mencía: Universidad Autónoma
Christophe Danelon: Delft University of Technology

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Replication of DNA-encoded information and its conversion into functional proteins are universal properties of life. In an effort toward the construction of a synthetic minimal cell, we implement here the DNA replication machinery of the Φ29 virus in a cell-free gene expression system. Amplification of a linear DNA template by self-encoded, de novo synthesized Φ29 proteins is demonstrated. Complete information transfer is confirmed as the copied DNA can serve as a functional template for gene expression, which can be seen as an autocatalytic DNA replication cycle. These results show how the central dogma of molecular biology can be reconstituted and form a cycle in vitro. Finally, coupled DNA replication and gene expression is compartmentalized inside phospholipid vesicles providing the chassis for evolving functions in a prospective synthetic cell relying on the extant biology.

Date: 2018
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DOI: 10.1038/s41467-018-03926-1

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