Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA

Hai Zemmour, David Planer, Judith Magenheim, Joshua Moss, Daniel Neiman, Dan Gilon, Amit Korach, Benjamin Glaser, Ruth Shemer, Giora Landesberg () and Yuval Dor ()
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Hai Zemmour: The Hebrew University-Hadassah Medical School
David Planer: Hadassah-Hebrew University Medical Center
Judith Magenheim: The Hebrew University-Hadassah Medical School
Joshua Moss: The Hebrew University-Hadassah Medical School
Daniel Neiman: The Hebrew University-Hadassah Medical School
Dan Gilon: Hadassah-Hebrew University Medical Center
Amit Korach: Hadassah-Hebrew University Medical Center
Benjamin Glaser: Hadassah-Hebrew University Medical Center
Ruth Shemer: The Hebrew University-Hadassah Medical School
Giora Landesberg: Hadassah-Hebrew University Medical Center
Yuval Dor: The Hebrew University-Hadassah Medical School

Nature Communications, 2018, vol. 9, issue 1, 1-9

Abstract: Abstract Detection of cardiomyocyte death is crucial for the diagnosis and treatment of heart disease. Here we use comparative methylome analysis to identify genomic loci that are unmethylated specifically in cardiomyocytes, and develop these as biomarkers to quantify cardiomyocyte DNA in circulating cell-free DNA (cfDNA) derived from dying cells. Plasma of healthy individuals contains essentially no cardiomyocyte cfDNA, consistent with minimal cardiac turnover. Patients with acute ST-elevation myocardial infarction show a robust cardiac cfDNA signal that correlates with levels of troponin and creatine phosphokinase (CPK), including the expected elevation-decay dynamics following coronary angioplasty. Patients with sepsis have high cardiac cfDNA concentrations that strongly predict mortality, suggesting a major role of cardiomyocyte death in mortality from sepsis. A cfDNA biomarker for cardiomyocyte death may find utility in diagnosis and monitoring of cardiac pathologies and in the study of normal human cardiac physiology and development.

Date: 2018
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DOI: 10.1038/s41467-018-03961-y

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