Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress

Jiska Reest, Sergio Lilla, Liang Zheng (), Sara Zanivan () and Eyal Gottlieb ()
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Jiska Reest: Cancer Research UK Beatson Institute
Sergio Lilla: Cancer Research UK Beatson Institute
Liang Zheng: Cancer Research UK Beatson Institute
Sara Zanivan: Cancer Research UK Beatson Institute
Eyal Gottlieb: Cancer Research UK Beatson Institute

Nature Communications, 2018, vol. 9, issue 1, 1-16

Abstract: Abstract Reactive oxygen species (ROS) are increasingly recognised as important signalling molecules through oxidation of protein cysteine residues. Comprehensive identification of redox-regulated proteins and pathways is crucial to understand ROS-mediated events. Here, we present stable isotope cysteine labelling with iodoacetamide (SICyLIA), a mass spectrometry-based workflow to assess proteome-scale cysteine oxidation. SICyLIA does not require enrichment steps and achieves unbiased proteome-wide sensitivity. Applying SICyLIA to diverse cellular models and primary tissues provides detailed insights into thiol oxidation proteomes. Our results demonstrate that acute and chronic oxidative stress causes oxidation of distinct metabolic proteins, indicating that cysteine oxidation plays a key role in the metabolic adaptation to redox stress. Analysis of mouse kidneys identifies oxidation of proteins circulating in biofluids, through which cellular redox stress can affect whole-body physiology. Obtaining accurate peptide oxidation profiles from complex organs using SICyLIA holds promise for future analysis of patient-derived samples to study human pathologies.

Date: 2018
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DOI: 10.1038/s41467-018-04003-3

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