Quantifying absolute addressability in DNA origami with molecular resolution

Maximilian T. Strauss, Florian Schueder, Daniel Haas, Philipp C. Nickels and Ralf Jungmann ()
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Maximilian T. Strauss: Ludwig Maximilian University
Florian Schueder: Ludwig Maximilian University
Daniel Haas: Ludwig Maximilian University
Philipp C. Nickels: Ludwig Maximilian University
Ralf Jungmann: Ludwig Maximilian University

Nature Communications, 2018, vol. 9, issue 1, 1-7

Abstract: Abstract Self-assembled DNA nanostructures feature an unprecedented addressability with sub-nanometer precision and accuracy. This addressability relies on the ability to attach functional entities to single DNA strands in these structures. The efficiency of this attachment depends on two factors: incorporation of the strand of interest and accessibility of this strand for downstream modification. Here we use DNA-PAINT super-resolution microscopy to quantify both incorporation and accessibility of all individual strands in DNA origami with molecular resolution. We find that strand incorporation strongly correlates with the position in the structure, ranging from a minimum of 48% on the edges to a maximum of 95% in the center. Our method offers a direct feedback for the rational refinement of the design and assembly process of DNA nanostructures and provides a long sought-after quantitative explanation for efficiencies of DNA-based nanomachines.

Date: 2018
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DOI: 10.1038/s41467-018-04031-z

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