Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Etten, Michael G Rossmann, Zihe Rao and Xinzheng Zhang ()
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Dongjie Zhu: Chinese Academy of Sciences
Xiangxi Wang: Chinese Academy of Sciences
Qianglin Fang: Purdue University
James L Etten: University of Nebraska
Michael G Rossmann: Purdue University
Zihe Rao: Chinese Academy of Sciences
Xinzheng Zhang: Chinese Academy of Sciences

Nature Communications, 2018, vol. 9, issue 1, 1-7

Abstract: Abstract The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.

Date: 2018
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DOI: 10.1038/s41467-018-04051-9

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