Supramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging

Kyung Lock Kim, Gihyun Sung, Jaehwan Sim, James Murray, Meng Li, Ara Lee, Annadka Shrinidhi, Kyeng Min Park () and Kimoon Kim ()
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Kyung Lock Kim: Institute for Basic Science (IBS)
Gihyun Sung: Pohang University of Science and Technology (POSTECH)
Jaehwan Sim: Pohang University of Science and Technology (POSTECH)
James Murray: Institute for Basic Science (IBS)
Meng Li: Institute for Basic Science (IBS)
Ara Lee: Pohang University of Science and Technology (POSTECH)
Annadka Shrinidhi: Institute for Basic Science (IBS)
Kyeng Min Park: Institute for Basic Science (IBS)
Kimoon Kim: Institute for Basic Science (IBS)

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract Here we report ultrastable synthetic binding pairs between cucurbit[7]uril (CB[7]) and adamantyl- (AdA) or ferrocenyl-ammonium (FcA) as a supramolecular latching system for protein imaging, overcoming the limitations of protein-based binding pairs. Cyanine 3-conjugated CB[7] (Cy3-CB[7]) can visualize AdA- or FcA-labeled proteins to provide clear fluorescence images for accurate and precise analysis of proteins. Furthermore, controllability of the system is demonstrated by treating with a stronger competitor guest. At low temperature, this allows us to selectively detach Cy3-CB[7] from guest-labeled proteins on the cell surface, while leaving Cy3-CB[7] latched to the cytosolic proteins for spatially conditional visualization of target proteins. This work represents a non-protein-based bioimaging tool which has inherent advantages over the widely used protein-based techniques, thereby demonstrating the great potential of this synthetic system.

Date: 2018
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DOI: 10.1038/s41467-018-04161-4

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