Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state

Mohammad U. Zahid, Liang Ma, Sung Jun Lim and Andrew M. Smith ()
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Mohammad U. Zahid: University of Illinois at Urbana-Champaign
Liang Ma: University of Illinois at Urbana-Champaign
Sung Jun Lim: University of Illinois at Urbana-Champaign
Andrew M. Smith: University of Illinois at Urbana-Champaign

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract Inefficient delivery of macromolecules and nanoparticles to intracellular targets is a major bottleneck in drug delivery, genetic engineering, and molecular imaging. Here we apply live-cell single-quantum-dot imaging and tracking to analyze and classify nanoparticle states after intracellular delivery. By merging trajectory diffusion parameters with brightness measurements, multidimensional analysis reveals distinct and heterogeneous populations that are indistinguishable using single parameters alone. We derive new quantitative metrics of particle loading, cluster distribution, and vesicular release in single cells, and evaluate intracellular nanoparticles with diverse surfaces following osmotic delivery. Surface properties have a major impact on cell uptake, but little impact on the absolute cytoplasmic numbers. A key outcome is that stable zwitterionic surfaces yield uniform cytosolic behavior, ideal for imaging agents. We anticipate that this combination of quantum dots and single-particle tracking can be widely applied to design and optimize next-generation imaging probes, nanoparticle therapeutics, and biologics.

Date: 2018
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DOI: 10.1038/s41467-018-04185-w

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