Single molecule fate of HIV-1 envelope reveals late-stage viral lattice incorporation

Carmen A. Buttler, Nairi Pezeshkian, Melissa V. Fernandez, Jesse Aaron, Sofya Norman, Eric O. Freed and Schuyler B. van Engelenburg ()
Additional contact information
Carmen A. Buttler: University of Denver
Nairi Pezeshkian: University of Denver
Melissa V. Fernandez: National Cancer Institute
Jesse Aaron: Howard Hughes Medical Institute
Sofya Norman: University of Denver
Eric O. Freed: National Cancer Institute
Schuyler B. van Engelenburg: University of Denver

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Human immunodeficiency virus type 1 (HIV-1) assembly occurs on the inner leaflet of the host cell plasma membrane, incorporating the essential viral envelope glycoprotein (Env) within a budding lattice of HIV-1 Gag structural proteins. The mechanism by which Env incorporates into viral particles remains poorly understood. To determine the mechanism of recruitment of Env to assembly sites, we interrogate the subviral angular distribution of Env on cell-associated virus using multicolor, three-dimensional (3D) superresolution microscopy. We demonstrate that, in a manner dependent on cell type and on the long cytoplasmic tail of Env, the distribution of Env is biased toward the necks of cell-associated particles. We postulate that this neck-biased distribution is regulated by vesicular retention and steric complementarity of Env during independent Gag lattice formation.

Date: 2018
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-018-04220-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04220-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-04220-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().