Regulatory protein SrpA controls phage infection and core cellular processes in Pseudomonas aeruginosa

Jiajia You, Li Sun, Xiaojing Yang, Xuewei Pan, Zhiwei Huang, Xixi Zhang, Mengxin Gong, Zheng Fan, Lingyan Li, Xiaoli Cui, Zhaoyuan Jing, Shouguang Jin, Zhiming Rao (), Weihui Wu () and Hongjiang Yang ()
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Jiajia You: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Li Sun: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Xiaojing Yang: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Xuewei Pan: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Zhiwei Huang: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Xixi Zhang: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Mengxin Gong: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Zheng Fan: College of Life Sciences, Nankai University
Lingyan Li: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Xiaoli Cui: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Zhaoyuan Jing: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology
Shouguang Jin: College of Life Sciences, Nankai University
Zhiming Rao: School of Biotechnology, Jiangnan University
Weihui Wu: College of Life Sciences, Nankai University
Hongjiang Yang: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract Our understanding of the molecular mechanisms behind bacteria-phage interactions remains limited. Here we report that a small protein, SrpA, controls core cellular processes in response to phage infection and environmental signals in Pseudomonas aeruginosa. We show that SrpA is essential for efficient genome replication of phage K5, and controls transcription by binding to a palindromic sequence upstream of the phage RNA polymerase gene. We identify potential SrpA-binding sites in 66 promoter regions across the P. aeruginosa genome, and experimentally validate direct binding of SrpA to some of these sites. Using transcriptomics and further experiments, we show that SrpA, directly or indirectly, regulates many cellular processes including cell motility, chemotaxis, biofilm formation, pyocyanin synthesis and protein secretion, as well as virulence in a Caenorhabditis elegans model of infection. Further research on SrpA and similar proteins, which are widely present in many other bacteria, is warranted.

Date: 2018
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DOI: 10.1038/s41467-018-04232-6

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