Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks

Teppei Ebina, Yoshito Masamizu, Yasuhiro R. Tanaka, Akiya Watakabe, Reiko Hirakawa, Yuka Hirayama, Riichiro Hira, Shin-Ichiro Terada, Daisuke Koketsu, Kazuo Hikosaka, Hiroaki Mizukami, Atsushi Nambu, Erika Sasaki, Tetsuo Yamamori and Masanori Matsuzaki ()
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Teppei Ebina: The University of Tokyo
Yoshito Masamizu: The University of Tokyo
Yasuhiro R. Tanaka: The University of Tokyo
Akiya Watakabe: RIKEN Center for Brain Science
Reiko Hirakawa: National Institute for Basic Biology
Yuka Hirayama: The University of Tokyo
Riichiro Hira: National Institute for Basic Biology
Shin-Ichiro Terada: The University of Tokyo
Daisuke Koketsu: National Institute for Physiological Sciences
Kazuo Hikosaka: Kawasaki University of Medical Welfare
Hiroaki Mizukami: Jichi Medical University
Atsushi Nambu: SOKENDAI (The Graduate University for Advanced Studies)
Erika Sasaki: Central Institute for Experimental Animals
Tetsuo Yamamori: RIKEN Center for Brain Science
Masanori Matsuzaki: The University of Tokyo

Nature Communications, 2018, vol. 9, issue 1, 1-16

Abstract: Abstract Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution. However, marmosets have posed a challenge due to limited success in training on motor tasks. Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. After 2–5 months of training sessions, head-fixed marmosets can control a manipulandum to move a cursor to a target on a screen. We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions. In a two-target reaching task, some neurons show direction-selective activity over the training days. In a short-term force-field adaptation task, some neurons change their activity when the force field is on. Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition.

Date: 2018
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DOI: 10.1038/s41467-018-04286-6

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