Structural and functional analysis of mRNA export regulation by the nuclear pore complex

Daniel H. Lin, Ana R. Correia, Sarah W. Cai, Ferdinand M. Huber, Claudia A. Jette and André Hoelz ()
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Daniel H. Lin: California Institute of Technology
Ana R. Correia: California Institute of Technology
Sarah W. Cai: California Institute of Technology
Ferdinand M. Huber: California Institute of Technology
Claudia A. Jette: California Institute of Technology
André Hoelz: California Institute of Technology

Nature Communications, 2018, vol. 9, issue 1, 1-19

Abstract: Abstract The nuclear pore complex (NPC) controls the passage of macromolecules between the nucleus and cytoplasm, but how the NPC directly participates in macromolecular transport remains poorly understood. In the final step of mRNA export, the DEAD-box helicase DDX19 is activated by the nucleoporins Gle1, Nup214, and Nup42 to remove Nxf1•Nxt1 from mRNAs. Here, we report crystal structures of Gle1•Nup42 from three organisms that reveal an evolutionarily conserved binding mode. Biochemical reconstitution of the DDX19 ATPase cycle establishes that human DDX19 activation does not require IP6, unlike its fungal homologs, and that Gle1 stability affects DDX19 activation. Mutations linked to motor neuron diseases cause decreased Gle1 thermostability, implicating nucleoporin misfolding as a disease determinant. Crystal structures of human Gle1•Nup42•DDX19 reveal the structural rearrangements in DDX19 from an auto-inhibited to an RNA-binding competent state. Together, our results provide the foundation for further mechanistic analyses of mRNA export in humans.

Date: 2018
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DOI: 10.1038/s41467-018-04459-3

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