CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge () and Jan Michiels ()
Additional contact information
Toon Swings: KU Leuven - University of Leuven
David C. Marciano: Baylor College of Medicine
Benu Atri: Baylor College of Medicine
Rachel E. Bosserman: McGovern Medical School
Chen Wang: Baylor College of Medicine
Marlies Leysen: KU Leuven - University of Leuven
Camille Bonte: KU Leuven - University of Leuven
Thomas Schalck: KU Leuven - University of Leuven
Ian Furey: Baylor College of Medicine
Bram Van den Bergh: KU Leuven - University of Leuven
Natalie Verstraeten: KU Leuven - University of Leuven
Peter J. Christie: McGovern Medical School
Christophe Herman: Baylor College of Medicine
Olivier Lichtarge: Baylor College of Medicine
Jan Michiels: KU Leuven - University of Leuven

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

Date: 2018
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DOI: 10.1038/s41467-018-04651-5

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