Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta

Chloé S. Baron, Lennart Kester, Anna Klaus, Jean-Charles Boisset, Roshana Thambyrajah, Laurent Yvernogeau, Valérie Kouskoff, Georges Lacaud, Alexander Oudenaarden and Catherine Robin ()
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Chloé S. Baron: University Medical Center Utrecht
Lennart Kester: University Medical Center Utrecht
Anna Klaus: University Medical Center Utrecht
Jean-Charles Boisset: University Medical Center Utrecht
Roshana Thambyrajah: The University of Manchester
Laurent Yvernogeau: University Medical Center Utrecht
Valérie Kouskoff: The University of Manchester
Georges Lacaud: The University of Manchester
Alexander Oudenaarden: University Medical Center Utrecht
Catherine Robin: University Medical Center Utrecht

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Haematopoietic stem cells (HSCs) are generated from haemogenic endothelial (HE) cells via the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos. The molecular events controlling endothelial specification, endothelial-to-haematopoietic transition (EHT) and IAHC formation, as it occurs in vivo inside the aorta, are still poorly understood. To gain insight in these processes, we performed single-cell RNA-sequencing of non-HE cells, HE cells, cells undergoing EHT, IAHC cells, and whole IAHCs isolated from mouse embryo aortas. Our analysis identified the genes and transcription factor networks activated during the endothelial-to-haematopoietic switch and IAHC cell maturation toward an HSC fate. Our study provides an unprecedented complete resource to study in depth HSC generation in vivo. It will pave the way for improving HSC production in vitro to address the growing need for tailor-made HSCs to treat patients with blood-related disorders.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04893-3

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DOI: 10.1038/s41467-018-04893-3

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