SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix

Masatoshi Fukuda, Tatsuya Yoshizawa (), Md. Fazlul Karim, Shihab U. Sobuz, Wataru Korogi, Daiki Kobayasi, Hiroki Okanishi, Masayoshi Tasaki, Katsuhiko Ono, Tomohiro Sawa, Yoshifumi Sato, Mami Chirifu, Takeshi Masuda, Teruya Nakamura, Hironori Tanoue, Kazuhisa Nakashima, Yoshihiro Kobashigawa, Hiroshi Morioka, Eva Bober, Sumio Ohtsuki, Yuriko Yamagata, Yukio Ando, Yuichi Oike, Norie Araki, Shu Takeda, Hiroshi Mizuta and Kazuya Yamagata
Additional contact information
Masatoshi Fukuda: Kumamoto University
Tatsuya Yoshizawa: Kumamoto University
Md. Fazlul Karim: Kumamoto University
Shihab U. Sobuz: Kumamoto University
Wataru Korogi: Kumamoto University
Daiki Kobayasi: Kumamoto University
Hiroki Okanishi: Kumamoto University
Masayoshi Tasaki: Kumamoto University
Katsuhiko Ono: Kumamoto University
Tomohiro Sawa: Kumamoto University
Yoshifumi Sato: Kumamoto University
Mami Chirifu: Kumamoto University
Takeshi Masuda: Kumamoto University
Teruya Nakamura: Kumamoto University
Hironori Tanoue: Kumamoto University
Kazuhisa Nakashima: Tsurumi University School of Dental Medicine
Yoshihiro Kobashigawa: Kumamoto University
Hiroshi Morioka: Kumamoto University
Eva Bober: Max-Planck-Institute for Heart and Lung Research
Sumio Ohtsuki: Kumamoto University
Yuriko Yamagata: Kumamoto University
Yukio Ando: Kumamoto University
Yuichi Oike: Kumamoto University
Norie Araki: Kumamoto University
Shu Takeda: Tokyo Medical and Dental University
Hiroshi Mizuta: Kumamoto University
Kazuya Yamagata: Kumamoto University

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract SP7/Osterix (OSX) is a master regulatory transcription factor that activates a variety of genes during differentiation of osteoblasts. However, the influence of post-translational modifications on the regulation of its transactivation activity is largely unknown. Here, we report that sirtuins, which are NAD(+)-dependent deacylases, regulate lysine deacylation-mediated transactivation of OSX. Germline Sirt7 knockout mice develop severe osteopenia characterized by decreased bone formation and an increase of osteoclasts. Similarly, osteoblast-specific Sirt7 knockout mice showed attenuated bone formation. Interaction of SIRT7 with OSX leads to the activation of transactivation by OSX without altering its protein expression. Deacylation of lysine (K) 368 in the C-terminal region of OSX by SIRT7 promote its N-terminal transactivation activity. In addition, SIRT7-mediated deacylation of K368 also facilitates depropionylation of OSX by SIRT1, thereby increasing OSX transactivation activity. In conclusion, our findings suggest that SIRT7 has a critical role in bone formation by regulating acylation of OSX.

Date: 2018
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DOI: 10.1038/s41467-018-05187-4

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