Lipid droplet and peroxisome biogenesis occur at the same ER subdomains

Amit S. Joshi (), Benjamin Nebenfuehr, Vineet Choudhary, Prasanna Satpute-Krishnan, Tim P. Levine, Andy Golden and William A. Prinz ()
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Amit S. Joshi: National Institute of Diabetes and Digestive and Kidney Diseases, NIH
Benjamin Nebenfuehr: National Institute of Diabetes and Digestive and Kidney Diseases, NIH
Vineet Choudhary: National Institute of Diabetes and Digestive and Kidney Diseases, NIH
Prasanna Satpute-Krishnan: Uniformed Services, University of the Health Sciences
Tim P. Levine: University College London, Institute of Ophthalmology
Andy Golden: National Institute of Diabetes and Digestive and Kidney Diseases, NIH
William A. Prinz: National Institute of Diabetes and Digestive and Kidney Diseases, NIH

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Nascent lipid droplet (LD) formation occurs in the endoplasmic reticulum (ER) membrane but it is not known how sites of biogenesis are determined. We previously identified ER domains in S. cerevisiae containing the reticulon homology domain (RHD) protein Pex30 that are regions where preperoxisomal vesicles (PPVs) form. Here, we show that Pex30 domains are also sites where most nascent LDs form. Mature LDs usually remain associated with Pex30 subdomains, and the same Pex30 subdomain can simultaneously associate with a LD and a PPV or peroxisome. We find that in higher eukaryotes multiple C2 domain containing transmembrane protein (MCTP2) is similar to Pex30: it contains an RHD and resides in ER domains where most nascent LD biogenesis occurs and that often associate with peroxisomes. Together, these findings indicate that most LDs and PPVs form and remain associated with conserved ER subdomains, and suggest a link between LD and peroxisome biogenesis.

Date: 2018
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DOI: 10.1038/s41467-018-05277-3

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