Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum

Sihui Wang, Fatima-Zahra Idrissi, Martin Hermansson, Alexandra Grippa, Christer S. Ejsing and Pedro Carvalho ()
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Sihui Wang: Sir William Dunn School of Pathology, University of Oxford
Fatima-Zahra Idrissi: Centre for Genomic Regulation (CRG)
Martin Hermansson: University of Southern Denmark
Alexandra Grippa: Centre for Genomic Regulation (CRG)
Christer S. Ejsing: University of Southern Denmark
Pedro Carvalho: Sir William Dunn School of Pathology, University of Oxford

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Lipid droplets (LDs) and peroxisomes are ubiquitous organelles with central roles in eukaryotic cells. Although the mechanisms involved in biogenesis of these organelles remain elusive, both seem to require the endoplasmic reticulum (ER). Here we show that in yeast the ER budding of these structurally unrelated organelles has remarkably similar requirements and involves cooperation between Pex30 and the seipin complex. In the absence of these components, budding of both LDs and peroxisomes is inhibited, leading to the ER accumulation of their respective constituent molecules, such as triacylglycerols and peroxisomal membrane proteins, whereas COPII vesicle formation remains unaffected. This phenotype can be reversed by remodeling ER phospholipid composition highlighting a key function of these lipids in organelle biogenesis. We propose that seipin and Pex30 act in concert to organize membrane domains permissive for organelle budding, and that may have a lipid composition distinct from the bulk ER.

Date: 2018
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DOI: 10.1038/s41467-018-05278-2

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