A reliable murine model of bone metastasis by injecting cancer cells through caudal arteries

Takahiro Kuchimaru, Naoya Kataoka, Kenji Nakagawa, Tatsuhiro Isozaki, Hitomi Miyabara, Misa Minegishi, Tetsuya Kadonosono and Shinae Kizaka-Kondoh ()
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Takahiro Kuchimaru: Tokyo Institute of Technology
Naoya Kataoka: Tokyo Institute of Technology
Kenji Nakagawa: Tokyo Institute of Technology
Tatsuhiro Isozaki: Tokyo Institute of Technology
Hitomi Miyabara: Tokyo Institute of Technology
Misa Minegishi: Tokyo Institute of Technology
Tetsuya Kadonosono: Tokyo Institute of Technology
Shinae Kizaka-Kondoh: Tokyo Institute of Technology

Nature Communications, 2018, vol. 9, issue 1, 1-7

Abstract: Abstract Although the current murine model of bone metastasis using intracardiac (IC) injection successfully recapitulates the process of bone metastasis, further progress in the study of bone metastasis requires a new model to circumvent some limitations of this model. Here, we present a new murine model of bone metastasis achieved by injecting cancer cells through the intra-caudal arterial (CA). This model does not require high technical proficiency, predominantly delivers cancer cells to bone marrow of hind limbs with much higher efficiency than IC injection, and greatly shortens the period of overt bone metastasis development. Moreover, CA injection barely causes acute death of mice, enabling us to inject a larger number of cancer cells to further accelerate the development of bone metastasis with a wide variety of cell lines. Our model may open a new avenue for understanding the bone metastatic processes and development of drugs preventing bone metastasis and recurrence.

Date: 2018
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DOI: 10.1038/s41467-018-05366-3

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