Size control in mammalian cells involves modulation of both growth rate and cell cycle duration

Clotilde Cadart, Sylvain Monnier, Jacopo Grilli, Pablo J. Sáez, Nishit Srivastava, Rafaele Attia, Emmanuel Terriac, Buzz Baum, Marco Cosentino-Lagomarsino () and Matthieu Piel ()
Additional contact information
Clotilde Cadart: PSL Research University, CNRS, UMR 144
Sylvain Monnier: PSL Research University, CNRS, UMR 144
Jacopo Grilli: University of Chicago
Pablo J. Sáez: PSL Research University, CNRS, UMR 144
Nishit Srivastava: PSL Research University, CNRS, UMR 144
Rafaele Attia: PSL Research University, CNRS, UMR 144
Emmanuel Terriac: PSL Research University, CNRS, UMR 144
Buzz Baum: MRC Laboratory for Molecular Cell Biology, UCL
Marco Cosentino-Lagomarsino: Sorbonne Universités, Université Pierre et Marie Curie
Matthieu Piel: PSL Research University, CNRS, UMR 144

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on various mammalian cell lines and primary human cells. We find that, in a majority of cell types, the volume added across the cell cycle shows little or no correlation to cell birth size, a homeostatic behavior called “adder”. This behavior involves modulation of G1 or S-G2 duration and modulation of growth rate. The precise combination of these mechanisms depends on the cell type and the growth condition. We have developed a mathematical framework to compare size homeostasis in datasets ranging from bacteria to mammalian cells. This reveals that a near-adder behavior is the most common type of size control and highlights the importance of growth rate modulation to size control in mammalian cells.

Date: 2018
References: Add references at CitEc
Citations: View citations in EconPapers (5)

Downloads: (external link)
https://www.nature.com/articles/s41467-018-05393-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05393-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-05393-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().