Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes

Ryan C. Ransom, Deshka S. Foster, Ankit Salhotra, Ruth Ellen Jones, Clement D. Marshall, Tripp Leavitt, Matthew P. Murphy, Alessandra L. Moore, Charles P. Blackshear, Elizabeth A. Brett, Derrick C. Wan and Michael T. Longaker ()
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Ryan C. Ransom: Stanford University School of Medicine
Deshka S. Foster: Stanford University School of Medicine
Ankit Salhotra: Stanford University School of Medicine
Ruth Ellen Jones: Stanford University School of Medicine
Clement D. Marshall: Stanford University School of Medicine
Tripp Leavitt: Stanford University School of Medicine
Matthew P. Murphy: Stanford University School of Medicine
Alessandra L. Moore: Stanford University School of Medicine
Charles P. Blackshear: Stanford University School of Medicine
Elizabeth A. Brett: Stanford University School of Medicine
Derrick C. Wan: Stanford University School of Medicine
Michael T. Longaker: Stanford University School of Medicine

Nature Communications, 2018, vol. 9, issue 1, 1-8

Abstract: Abstract Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreERT2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.

Date: 2018
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DOI: 10.1038/s41467-018-05436-6

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