Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe

Troy F. Langford, Beijing K. Huang, Joseph B. Lim, Sun Jin Moon and Hadley D. Sikes ()
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Troy F. Langford: Massachusetts Institute of Technology
Beijing K. Huang: Massachusetts Institute of Technology
Joseph B. Lim: Massachusetts Institute of Technology
Sun Jin Moon: Massachusetts Institute of Technology
Hadley D. Sikes: Massachusetts Institute of Technology

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Redox cancer therapeutics target the increased reliance on intracellular antioxidant systems and enhanced susceptibility to oxidant-induced stress of some cancer cells compared to normal cells. Many of these therapeutics are thought to perturb intracellular levels of the oxidant hydrogen peroxide (H2O2), a signaling molecule that modulates a number of different processes in human cells. However, fluorescent probes for this species remain limited in their ability to detect the small perturbations induced during successful treatments. We report a fluorescent sensor based upon human peroxiredoxin-2, which acts as the natural indicator of small H2O2 fluctuations in human cells. The new probe reveals peroxide-induced oxidation in human cells below the detection limit of current probes, as well as peroxiredoxin-2 oxidation caused by two different redox cancer therapeutics in living cells. This capability will be useful in elucidating the mechanism of current redox-based therapeutics and in developing new ones.

Date: 2018
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DOI: 10.1038/s41467-018-05557-y

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