Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding

Emma Lång, Anna Połeć, Anna Lång, Marijke Valk, Pernille Blicher, Alexander D. Rowe, Kim A. Tønseth, Catherine J. Jackson, Tor P. Utheim, Liesbeth M. C. Janssen, Jens Eriksson and Stig Ove Bøe ()
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Emma Lång: Oslo University Hospital
Anna Połeć: Oslo University Hospital
Anna Lång: Oslo University Hospital
Marijke Valk: Eindhoven University of Technology
Pernille Blicher: University of Oslo
Alexander D. Rowe: Oslo University Hospital
Kim A. Tønseth: Oslo University Hospital
Catherine J. Jackson: Oslo University Hospital
Tor P. Utheim: Oslo University Hospital
Liesbeth M. C. Janssen: Eindhoven University of Technology
Jens Eriksson: Oslo University Hospital
Stig Ove Bøe: Oslo University Hospital

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Epithelial sheet spreading is a fundamental cellular process that must be coordinated with cell division and differentiation to restore tissue integrity. Here we use consecutive serum deprivation and re-stimulation to reconstruct biphasic collective migration and proliferation in cultured sheets of human keratinocytes. In this system, a burst of long-range coordinated locomotion is rapidly generated throughout the cell sheet in the absence of wound edges. Migrating cohorts reach correlation lengths of several millimeters and display dependencies on epidermal growth factor receptor-mediated signaling, self-propelled polarized migration, and a G1/G0 cell cycle environment. The migration phase is temporally and spatially aligned with polarized cell divisions characterized by pre-mitotic nuclear migration to the cell front and asymmetric partitioning of nuclear promyelocytic leukemia bodies and lysosomes to opposite daughter cells. This study investigates underlying mechanisms contributing to the stark contrast between cells in a static quiescent state compared to the long-range coordinated collective migration seen in contact with blood serum.

Date: 2018
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DOI: 10.1038/s41467-018-05578-7

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