Architecture of the native major royal jelly protein 1 oligomer

Wenli Tian, Min Li, Huiyuan Guo, Wenjun Peng, Xiaofeng Xue, Yifan Hu, Yang Liu, Yazhou Zhao, Xiaoming Fang, Kai Wang, Xiuting Li, Yufeng Tong, Michael A. Conlon, Wei Wu, Fazheng Ren and Zhongzhou Chen ()
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Wenli Tian: China Agricultural University
Min Li: China Agricultural University
Huiyuan Guo: China Agricultural University
Wenjun Peng: Chinese Academy of Agricultural Sciences
Xiaofeng Xue: Chinese Academy of Agricultural Sciences
Yifan Hu: Chinese Academy of Agricultural Sciences
Yang Liu: China Agricultural University
Yazhou Zhao: Chinese Academy of Agricultural Sciences
Xiaoming Fang: Chinese Academy of Agricultural Sciences
Kai Wang: Chinese Academy of Agricultural Sciences
Xiuting Li: Beijing Technology and Business University
Yufeng Tong: University of Toronto
Michael A. Conlon: CSIRO Health and Biosecurity
Wei Wu: China Agricultural University
Fazheng Ren: China Agricultural University
Zhongzhou Chen: China Agricultural University

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Honeybee caste development is nutritionally regulated by royal jelly (RJ). Major royal jelly protein 1 (MRJP1), the most abundant glycoprotein among soluble royal jelly proteins, plays pivotal roles in honeybee nutrition and larvae development, and exhibits broad pharmacological activities in humans. However, its structure has long remained unknown. Herein, we identify and report a 16-molecule architecture of native MRJP1 oligomer containing four MRJP1, four apisimin, and eight unanticipated 24-methylenecholesterol molecules at 2.65 Å resolution. MRJP1 has a unique six-bladed β-propeller fold with three disulfide bonds, and it interacts with apisimin mainly by hydrophobic interaction. Every four 24-methylenecholesterol molecules are packaged by two MRJP1 and two apisimin molecules. This assembly dimerizes to form an H-shaped MRJP14-apisimin4-24-methylenecholesterol8 complex via apisimin in a conserved and pH-dependent fashion. Our findings offer a structural basis for understanding the pharmacological effects of MRJPs and 24-methylenecholesterol, and provide insights into their unique physiological roles in bees.

Date: 2018
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DOI: 10.1038/s41467-018-05619-1

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