Revealing the mechanism for covalent inhibition of glycoside hydrolases by carbasugars at an atomic level

Weiwu Ren, Robert Pengelly, Marco Farren-Dai, Saeideh Shamsi Kazem Abadi, Verena Oehler, Oluwafemi Akintola, Jason Draper, Michael Meanwell, Saswati Chakladar, Katarzyna Świderek, Vicent Moliner, Robert Britton (), Tracey M. Gloster () and Andrew J. Bennet ()
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Weiwu Ren: Simon Fraser University
Robert Pengelly: University of St Andrews
Marco Farren-Dai: Simon Fraser University
Saeideh Shamsi Kazem Abadi: Simon Fraser University
Verena Oehler: University of St Andrews
Oluwafemi Akintola: Simon Fraser University
Jason Draper: Simon Fraser University
Michael Meanwell: Simon Fraser University
Saswati Chakladar: Simon Fraser University
Katarzyna Świderek: Universitat Jaume I
Vicent Moliner: Universitat Jaume I
Robert Britton: Simon Fraser University
Tracey M. Gloster: University of St Andrews
Andrew J. Bennet: Simon Fraser University

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Mechanism-based glycoside hydrolase inhibitors are carbohydrate analogs that mimic the natural substrate’s structure. Their covalent bond formation with the glycoside hydrolase makes these compounds excellent tools for chemical biology and potential drug candidates. Here we report the synthesis of cyclohexene-based α-galactopyranoside mimics and the kinetic and structural characterization of their inhibitory activity toward an α-galactosidase from Thermotoga maritima (TmGalA). By solving the structures of several enzyme-bound species during mechanism-based covalent inhibition of TmGalA, we show that the Michaelis complexes for intact inhibitor and product have half-chair (2H3) conformations for the cyclohexene fragment, while the covalently linked intermediate adopts a flattened half-chair (2H3) conformation. Hybrid QM/MM calculations confirm the structural and electronic properties of the enzyme-bound species and provide insight into key interactions in the enzyme-active site. These insights should stimulate the design of mechanism-based glycoside hydrolase inhibitors with tailored chemical properties.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05702-7

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DOI: 10.1038/s41467-018-05702-7

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