Biased genome editing using the local accumulation of DSB repair molecules system

Shota Nakade, Keiji Mochida, Atsushi Kunii, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, Tetsushi Sakuma () and Takashi Yamamoto ()
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Shota Nakade: Hiroshima University
Keiji Mochida: Hiroshima University
Atsushi Kunii: Hiroshima University
Kazuki Nakamae: Hiroshima University
Tomomi Aida: Tokyo Medical and Dental University (TMDU)
Kohichi Tanaka: Tokyo Medical and Dental University (TMDU)
Naoaki Sakamoto: Hiroshima University
Tetsushi Sakuma: Hiroshima University
Takashi Yamamoto: Hiroshima University

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract Selective genome editing such as gene knock-in has recently been achieved by administration of chemical enhancer or inhibitor of particular DNA double-strand break (DSB) repair pathways, as well as overexpression of pathway-specific genes. In this study, we attempt to enhance the efficiency further to secure robust gene knock-ins, by using the local accumulation of DSB repair molecules (LoAD) system. We identify CtIP as a strong enhancer of microhomology-mediated end-joining (MMEJ) repair by genetic screening, and show the knock-in-enhancing effect of CtIP LoADing. Next-generation sequencing reveals that CtIP LoADing highly increases the frequency of MMEJ-mediated integration. Selection-free, simultaneous triple gene knock-ins are also achieved with the CtIP-LoADing strategy. Moreover, by replacing the LoADing molecules and targeting strategies, this system can be applied for other specific genome engineering purposes, such as introducing longer deletions for gene disruption, independently introducing multiple mutations without chromosomal deletion, and efficiently incorporating a single-stranded oligodeoxynucleotide donor.

Date: 2018
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DOI: 10.1038/s41467-018-05773-6

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