Monolayer surface chemistry enables 2-colour single molecule localisation microscopy of adhesive ligands and adhesion proteins

Xun Lu, Philip R. Nicovich, Manchen Zhao, Daniel J. Nieves, Mahdie Mollazade, S. R. C. Vivekchand, Katharina Gaus () and J. Justin Gooding ()
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Xun Lu: University of New South Wales
Philip R. Nicovich: University of New South Wales
Manchen Zhao: University of New South Wales
Daniel J. Nieves: University of New South Wales
Mahdie Mollazade: University of New South Wales
S. R. C. Vivekchand: University of New South Wales
Katharina Gaus: University of New South Wales
J. Justin Gooding: University of New South Wales

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract Nanofabricated and nanopatterned surfaces have revealed the sensitivity of cell adhesion to nanoscale variations in the spacing of adhesive ligands such as the tripeptide arginine-glycine-aspartic acid (RGD). To date, surface characterisation and cell adhesion are often examined in two separate experiments so that the localisation of ligands and adhesion proteins cannot be combined in the same image. Here we developed self-assembled monolayer chemistry for indium tin oxide (ITO) surfaces for single molecule localisation microscopy (SMLM). Cell adhesion and spreading were sensitive to average RGD spacing. At low average RGD spacing, a threshold exists of 0.8 RGD peptides per µm2 that tether cells to the substratum but this does not enable formation of focal adhesions. These findings suggest that cells can sense and engage single adhesive ligands but ligand clustering is required for cell spreading. Thus, our data reveal subtle differences in adhesion biology that may be obscured in ensemble measurements.

Date: 2018
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DOI: 10.1038/s41467-018-05837-7

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