Vertebrate myosin 1d regulates left–right organizer morphogenesis and laterality

Manush Saydmohammed (), Hisato Yagi, Michael Calderon, Madeline J. Clark, Timothy Feinstein, Ming Sun, Donna B. Stolz, Simon C. Watkins, Jeffrey D. Amack, Cecilia W. Lo and Michael Tsang ()
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Manush Saydmohammed: University of Pittsburgh
Hisato Yagi: University of Pittsburgh
Michael Calderon: University of Pittsburgh
Madeline J. Clark: State University of New York Upstate Medical University
Timothy Feinstein: University of Pittsburgh
Ming Sun: University of Pittsburgh
Donna B. Stolz: University of Pittsburgh
Simon C. Watkins: University of Pittsburgh
Jeffrey D. Amack: State University of New York Upstate Medical University
Cecilia W. Lo: University of Pittsburgh
Michael Tsang: University of Pittsburgh

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract Establishing left–right asymmetry is a fundamental process essential for arrangement of visceral organs during development. In vertebrates, motile cilia-driven fluid flow in the left–right organizer (LRO) is essential for initiating symmetry breaking event. Here, we report that myosin 1d (myo1d) is essential for establishing left–right asymmetry in zebrafish. Using super-resolution microscopy, we show that the zebrafish LRO, Kupffer’s vesicle (KV), fails to form a spherical lumen and establish proper unidirectional flow in the absence of myo1d. This process requires directed vacuolar trafficking in KV epithelial cells. Interestingly, the vacuole transporting function of zebrafish Myo1d can be substituted by myosin1C derived from an ancient eukaryote, Acanthamoeba castellanii, where it regulates the transport of contractile vacuoles. Our findings reveal an evolutionary conserved role for an unconventional myosin in vacuole trafficking, lumen formation, and determining laterality.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05866-2

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DOI: 10.1038/s41467-018-05866-2

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