Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains

Laura Pascual-Reguant, Enrique Blanco, Silvia Galan, François Dily, Yasmina Cuartero, Gemma Serra-Bardenys, Valerio Carlo, Ane Iturbide, Joan Pau Cebrià-Costa, Lara Nonell, Antonio García Herreros, Luciano Croce, Marc A. Marti-Renom and Sandra Peiró ()
Additional contact information
Laura Pascual-Reguant: Vall d’Hebron Institute of Oncology
Enrique Blanco: The Barcelona Institute of Science and Technology
Silvia Galan: The Barcelona Institute of Science and Technology
François Dily: The Barcelona Institute of Science and Technology
Yasmina Cuartero: The Barcelona Institute of Science and Technology
Gemma Serra-Bardenys: Vall d’Hebron Institute of Oncology
Valerio Carlo: The Barcelona Institute of Science and Technology
Ane Iturbide: Institute of Epigenetics and Stem Cells
Joan Pau Cebrià-Costa: Vall d’Hebron Institute of Oncology
Lara Nonell: Servei d’Anàlisi de Microarrays Institut Hospital del Mar d’Investigacions Mèdiques
Antonio García Herreros: Universitat Pompeu Fabra (UPF)
Luciano Croce: The Barcelona Institute of Science and Technology
Marc A. Marti-Renom: The Barcelona Institute of Science and Technology
Sandra Peiró: Vall d’Hebron Institute of Oncology

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Lamins (A/C and B) are major constituents of the nuclear lamina (NL). Structurally conserved lamina-associated domains (LADs) are formed by genomic regions that contact the NL. Lamins are also found in the nucleoplasm, with a yet unknown function. Here we map the genome-wide localization of lamin B1 in an euchromatin-enriched fraction of the mouse genome and follow its dynamics during the epithelial-to-mesenchymal transition (EMT). Lamin B1 associates with actively expressed and open euchromatin regions, forming dynamic euchromatin lamin B1-associated domains (eLADs) of about 0.3 Mb. Hi-C data link eLADs to the 3D organization of the mouse genome during EMT and correlate lamin B1 enrichment at topologically associating domain (TAD) borders with increased border strength. Having reduced levels of lamin B1 alters the EMT transcriptional signature and compromises the acquisition of mesenchymal traits. Thus, during EMT, the process of genome reorganization in mouse involves dynamic changes in eLADs.

Date: 2018
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-018-05912-z Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05912-z

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-05912-z

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().