CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts

Melvin Noe Gonzalez, Shigeo Sato, Chieri Tomomori-Sato, Joan W. Conaway and Ronald C. Conaway ()
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Melvin Noe Gonzalez: Stowers Institute for Medical Research
Shigeo Sato: Stowers Institute for Medical Research
Chieri Tomomori-Sato: Stowers Institute for Medical Research
Joan W. Conaway: Stowers Institute for Medical Research
Ronald C. Conaway: Stowers Institute for Medical Research

Nature Communications, 2018, vol. 9, issue 1, 1-13

Abstract: Abstract Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a “tethering” model for the mechanism of activation.

Date: 2018
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DOI: 10.1038/s41467-018-05923-w

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