Super-wide-field two-photon imaging with a micro-optical device moving in post-objective space

Shin-Ichiro Terada (), Kenta Kobayashi, Masamichi Ohkura, Junichi Nakai and Masanori Matsuzaki ()
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Shin-Ichiro Terada: The University of Tokyo
Kenta Kobayashi: National Institute for Physiological Sciences
Masamichi Ohkura: Saitama University
Junichi Nakai: Saitama University
Masanori Matsuzaki: The University of Tokyo

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract Wide-field imaging of neural activity at a cellular resolution is a current challenge in neuroscience. To address this issue, wide-field two-photon microscopy has been developed; however, the field size is limited by the objective size. Here, we develop a micro-opto-mechanical device that rotates within the post-objective space between the objective and brain tissue. Two-photon microscopy with this device enables sub-second sequential calcium imaging of left and right mouse sensory forelimb areas 6 mm apart. When imaging the rostral and caudal motor forelimb areas (RFA and CFA) 2 mm apart, we found high pairwise correlations in spontaneous activity between RFA and CFA neurons and between an RFA neuron and its putative axons in CFA. While mice performed a sound-triggered forelimb-movement task, the population activity between RFA and CFA covaried across trials, although the field-averaged activity was similar across trials. The micro-opto-mechanical device in the post-objective space provides a novel and flexible design to clarify the correlation structure between distant brain areas at subcellular and population levels.

Date: 2018
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DOI: 10.1038/s41467-018-06058-8

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