Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells

Angela M. Lager, Olivia G. Corradin, Jared M. Cregg, Matthew S. Elitt, H. Elizabeth Shick, Benjamin L. L. Clayton, Kevin C. Allan, Hannah E. Olsen, Mayur Madhavan and Paul J. Tesar ()
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Angela M. Lager: Case Western Reserve University School of Medicine
Olivia G. Corradin: Case Western Reserve University School of Medicine
Jared M. Cregg: Case Western Reserve University School of Medicine
Matthew S. Elitt: Case Western Reserve University School of Medicine
H. Elizabeth Shick: Case Western Reserve University School of Medicine
Benjamin L. L. Clayton: Case Western Reserve University School of Medicine
Kevin C. Allan: Case Western Reserve University School of Medicine
Hannah E. Olsen: Case Western Reserve University School of Medicine
Mayur Madhavan: Case Western Reserve University School of Medicine
Paul J. Tesar: Case Western Reserve University School of Medicine

Nature Communications, 2018, vol. 9, issue 1, 1-8

Abstract: Abstract Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype–phenotype relationships in oligodendrocytes.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06102-7

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DOI: 10.1038/s41467-018-06102-7

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