Megahertz serial crystallography

Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard; Schubert, Robin; Werner, Nadine; Abbey, Brian; Aepfelbacher, Martin; Adriano, Luigi; Allahgholi, Aschkan; Al-Qudami, Nasser; Andreasson, Jakob; Aplin, Steve; Awel, Salah; Ayyer, Kartik; Bajt, Saša; Barák, Imrich; Bari, Sadia; Bielecki, Johan; Botha, Sabine; Boukhelef, Djelloul; Brehm, Wolfgang; Brockhauser, Sandor; Cheviakov, Igor; Coleman, Matthew A.; Cruz-Mazo, Francisco; Danilevski, Cyril; Darmanin, Connie; Doak, R. Bruce; Domaracky, Martin; Dörner, Katerina; Du, Yang; Fangohr, Hans; Fleckenstein, Holger; Frank, Matthias; Fromme, Petra; Gañán-Calvo, Alfonso M.; Gevorkov, Yaroslav; Giewekemeyer, Klaus; Ginn, Helen Mary; Graafsma, Heinz; Graceffa, Rita; Greiffenberg, Dominic; Gumprecht, Lars; Göttlicher, Peter; Hajdu, Janos; Hauf, Steffen; Heymann, Michael; Holmes, Susannah; Horke, Daniel A.; Hunter, Mark S.; Imlau, Siegfried; Kaukher, Alexander; Kim, Yoonhee; Klyuev, Alexander; Knoška, Juraj; Kobe, Bostjan; Kuhn, Manuela; Kupitz, Christopher; Küpper, Jochen; Lahey-Rudolph, Janine Mia; Laurus, Torsten; Cong, Karoline; Letrun, Romain; Xavier, P. Lourdu; Maia, Luis; Maia, Filipe R. N. C.; Mariani, Valerio; Messerschmidt, Marc; Metz, Markus; Mezza, Davide; Michelat, Thomas; Mills, Grant; Monteiro, Diana C. F.; Morgan, Andrew; Mühlig, Kerstin; Munke, Anna; Münnich, Astrid; Nette, Julia; Nugent, Keith A.; Nuguid, Theresa; Orville, Allen M.; Pandey, Suraj; Pena, Gisel; Villanueva-Perez, Pablo; Poehlsen, Jennifer; Previtali, Gianpietro; Redecke, Lars; Riekehr, Winnie Maria; Rohde, Holger; Round, Adam; Safenreiter, Tatiana; Sarrou, Iosifina; Sato, Tokushi; Schmidt, Marius; Schmitt, Bernd; Schönherr, Robert; Schulz, Joachim; Sellberg, Jonas A.; Seibert, M. Marvin; Seuring, Carolin; Shelby, Megan L.; Shoeman, Robert L.; Sikorski, Marcin; Silenzi, Alessandro; Stan, Claudiu A.; Shi, Xintian; Stern, Stephan; Sztuk-Dambietz, Jola; Szuba, Janusz; Tolstikova, Aleksandra; Trebbin, Martin; Trunk, Ulrich; Vagovic, Patrik; Ve, Thomas; Weinhausen, Britta; White, Thomas A.; Wrona, Krzysztof; Xu, Chen; Yefanov, Oleksandr; Zatsepin, Nadia; Zhang, Jiaguo; Perbandt, Markus; Mancuso, Adrian P.; Betzel, Christian; Chapman, Henry; Barty, Anton

Megahertz serial crystallography

Max O. Wiedorn, Dominik Oberthür, Richard Bean, Robin Schubert, Nadine Werner, Brian Abbey, Martin Aepfelbacher, Luigi Adriano, Aschkan Allahgholi, Nasser Al-Qudami, Jakob Andreasson, Steve Aplin, Salah Awel, Kartik Ayyer, Saša Bajt, Imrich Barák, Sadia Bari, Johan Bielecki, Sabine Botha, Djelloul Boukhelef, Wolfgang Brehm, Sandor Brockhauser, Igor Cheviakov, Matthew A. Coleman, Francisco Cruz-Mazo, Cyril Danilevski, Connie Darmanin, R. Bruce Doak, Martin Domaracky, Katerina Dörner, Yang Du, Hans Fangohr, Holger Fleckenstein, Matthias Frank, Petra Fromme, Alfonso M. Gañán-Calvo, Yaroslav Gevorkov, Klaus Giewekemeyer, Helen Mary Ginn, Heinz Graafsma, Rita Graceffa, Dominic Greiffenberg, Lars Gumprecht, Peter Göttlicher, Janos Hajdu, Steffen Hauf, Michael Heymann, Susannah Holmes, Daniel A. Horke, Mark S. Hunter, Siegfried Imlau, Alexander Kaukher, Yoonhee Kim, Alexander Klyuev, Juraj Knoška, Bostjan Kobe, Manuela Kuhn, Christopher Kupitz, Jochen Küpper, Janine Mia Lahey-Rudolph, Torsten Laurus, Karoline Cong, Romain Letrun, P. Lourdu Xavier, Luis Maia, Filipe R. N. C. Maia, Valerio Mariani, Marc Messerschmidt, Markus Metz, Davide Mezza, Thomas Michelat, Grant Mills, Diana C. F. Monteiro, Andrew Morgan, Kerstin Mühlig, Anna Munke, Astrid Münnich, Julia Nette, Keith A. Nugent, Theresa Nuguid, Allen M. Orville, Suraj Pandey, Gisel Pena, Pablo Villanueva-Perez, Jennifer Poehlsen, Gianpietro Previtali, Lars Redecke, Winnie Maria Riekehr, Holger Rohde, Adam Round, Tatiana Safenreiter, Iosifina Sarrou, Tokushi Sato, Marius Schmidt, Bernd Schmitt, Robert Schönherr, Joachim Schulz, Jonas A. Sellberg, M. Marvin Seibert, Carolin Seuring, Megan L. Shelby, Robert L. Shoeman, Marcin Sikorski, Alessandro Silenzi, Claudiu A. Stan, Xintian Shi, Stephan Stern, Jola Sztuk-Dambietz, Janusz Szuba, Aleksandra Tolstikova, Martin Trebbin, Ulrich Trunk, Patrik Vagovic, Thomas Ve, Britta Weinhausen, Thomas A. White, Krzysztof Wrona, Chen Xu, Oleksandr Yefanov, Nadia Zatsepin, Jiaguo Zhang, Markus Perbandt, Adrian P. Mancuso, Christian Betzel, Henry Chapman () and Anton Barty ()
Additional contact information
Max O. Wiedorn: Deutsches Elektronen-Synchrotron DESY
Dominik Oberthür: Deutsches Elektronen-Synchrotron DESY
Richard Bean: European XFEL GmbH
Robin Schubert: Universität Hamburg
Nadine Werner: Universität Hamburg
Brian Abbey: La Trobe University
Martin Aepfelbacher: University Medical Center Hamburg-Eppendorf (UKE)
Luigi Adriano: Deutsches Elektronen-Synchrotron DESY
Aschkan Allahgholi: Deutsches Elektronen-Synchrotron DESY
Nasser Al-Qudami: European XFEL GmbH
Jakob Andreasson: Uppsala University
Steve Aplin: Deutsches Elektronen-Synchrotron DESY
Salah Awel: Deutsches Elektronen-Synchrotron DESY
Kartik Ayyer: Deutsches Elektronen-Synchrotron DESY
Saša Bajt: Deutsches Elektronen-Synchrotron DESY
Imrich Barák: SAS
Sadia Bari: Deutsches Elektronen-Synchrotron DESY
Johan Bielecki: European XFEL GmbH
Sabine Botha: Universität Hamburg
Djelloul Boukhelef: European XFEL GmbH
Wolfgang Brehm: Deutsches Elektronen-Synchrotron DESY
Sandor Brockhauser: European XFEL GmbH
Igor Cheviakov: University Medical Center Hamburg-Eppendorf (UKE)
Matthew A. Coleman: Lawrence Livermore National Laboratory
Francisco Cruz-Mazo: Universidad de Sevilla
Cyril Danilevski: European XFEL GmbH
Connie Darmanin: La Trobe University
R. Bruce Doak: Max Planck Institute for Medical Research
Martin Domaracky: Deutsches Elektronen-Synchrotron DESY
Katerina Dörner: European XFEL GmbH
Yang Du: Deutsches Elektronen-Synchrotron DESY
Hans Fangohr: European XFEL GmbH
Holger Fleckenstein: Deutsches Elektronen-Synchrotron DESY
Matthias Frank: Lawrence Livermore National Laboratory
Petra Fromme: Arizona State University
Alfonso M. Gañán-Calvo: Universidad de Sevilla
Yaroslav Gevorkov: Deutsches Elektronen-Synchrotron DESY
Klaus Giewekemeyer: European XFEL GmbH
Helen Mary Ginn: Division of Structural Biology, Headington
Heinz Graafsma: Deutsches Elektronen-Synchrotron DESY
Rita Graceffa: European XFEL GmbH
Dominic Greiffenberg: Paul Scherrer Institut
Lars Gumprecht: Deutsches Elektronen-Synchrotron DESY
Peter Göttlicher: Deutsches Elektronen-Synchrotron DESY
Janos Hajdu: Uppsala University
Steffen Hauf: European XFEL GmbH
Michael Heymann: Max Planck Institute of Biochemistry
Susannah Holmes: La Trobe University
Daniel A. Horke: Deutsches Elektronen-Synchrotron DESY
Mark S. Hunter: SLAC National Accelerator Laboratory
Siegfried Imlau: Deutsches Elektronen-Synchrotron DESY
Alexander Kaukher: European XFEL GmbH
Yoonhee Kim: European XFEL GmbH
Alexander Klyuev: Deutsches Elektronen-Synchrotron DESY
Juraj Knoška: Deutsches Elektronen-Synchrotron DESY
Bostjan Kobe: University of Queensland
Manuela Kuhn: Deutsches Elektronen-Synchrotron DESY
Christopher Kupitz: University of Wisconsin-Milwaukee
Jochen Küpper: Deutsches Elektronen-Synchrotron DESY
Janine Mia Lahey-Rudolph: Deutsches Elektronen-Synchrotron DESY
Torsten Laurus: Deutsches Elektronen-Synchrotron DESY
Karoline Cong: Universität Hamburg
Romain Letrun: European XFEL GmbH
P. Lourdu Xavier: Deutsches Elektronen-Synchrotron DESY
Luis Maia: European XFEL GmbH
Filipe R. N. C. Maia: Uppsala University
Valerio Mariani: Deutsches Elektronen-Synchrotron DESY
Marc Messerschmidt: European XFEL GmbH
Markus Metz: Deutsches Elektronen-Synchrotron DESY
Davide Mezza: Paul Scherrer Institut
Thomas Michelat: European XFEL GmbH
Grant Mills: European XFEL GmbH
Diana C. F. Monteiro: Universität Hamburg
Andrew Morgan: Deutsches Elektronen-Synchrotron DESY
Kerstin Mühlig: Uppsala University
Anna Munke: Uppsala University
Astrid Münnich: European XFEL GmbH
Julia Nette: Universität Hamburg
Keith A. Nugent: La Trobe University
Theresa Nuguid: Universität Hamburg
Allen M. Orville: Harwell Science and Innovation Campus
Suraj Pandey: University of Wisconsin-Milwaukee
Gisel Pena: Deutsches Elektronen-Synchrotron DESY
Pablo Villanueva-Perez: Deutsches Elektronen-Synchrotron DESY
Jennifer Poehlsen: Deutsches Elektronen-Synchrotron DESY
Gianpietro Previtali: European XFEL GmbH
Lars Redecke: University Medical Center Hamburg-Eppendorf (UKE)
Winnie Maria Riekehr: University of Lübeck
Holger Rohde: University Medical Center Hamburg-Eppendorf (UKE)
Adam Round: European XFEL GmbH
Tatiana Safenreiter: Deutsches Elektronen-Synchrotron DESY
Iosifina Sarrou: Deutsches Elektronen-Synchrotron DESY
Tokushi Sato: Deutsches Elektronen-Synchrotron DESY
Marius Schmidt: University of Wisconsin-Milwaukee
Bernd Schmitt: Paul Scherrer Institut
Robert Schönherr: University of Lübeck
Joachim Schulz: European XFEL GmbH
Jonas A. Sellberg: KTH Royal Institute of Technology
M. Marvin Seibert: Uppsala University
Carolin Seuring: Deutsches Elektronen-Synchrotron DESY
Megan L. Shelby: Lawrence Livermore National Laboratory
Robert L. Shoeman: Max Planck Institute for Medical Research
Marcin Sikorski: European XFEL GmbH
Alessandro Silenzi: European XFEL GmbH
Claudiu A. Stan: Rutgers University Newark
Xintian Shi: Paul Scherrer Institut
Stephan Stern: Deutsches Elektronen-Synchrotron DESY
Jola Sztuk-Dambietz: European XFEL GmbH
Janusz Szuba: European XFEL GmbH
Aleksandra Tolstikova: Deutsches Elektronen-Synchrotron DESY
Martin Trebbin: Universität Hamburg
Ulrich Trunk: Deutsches Elektronen-Synchrotron DESY
Patrik Vagovic: Deutsches Elektronen-Synchrotron DESY
Thomas Ve: Griffith University
Britta Weinhausen: European XFEL GmbH
Thomas A. White: Deutsches Elektronen-Synchrotron DESY
Krzysztof Wrona: European XFEL GmbH
Chen Xu: European XFEL GmbH
Oleksandr Yefanov: Deutsches Elektronen-Synchrotron DESY
Nadia Zatsepin: Arizona State University
Jiaguo Zhang: Paul Scherrer Institut
Markus Perbandt: Universität Hamburg
Adrian P. Mancuso: European XFEL GmbH
Christian Betzel: Universität Hamburg
Henry Chapman: Deutsches Elektronen-Synchrotron DESY
Anton Barty: Deutsches Elektronen-Synchrotron DESY

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06156-7

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DOI: 10.1038/s41467-018-06156-7

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