Strobilurin biosynthesis in Basidiomycete fungi

Risa Nofiani, Kate Mattos-Shipley, Karen E. Lebe, Li-Chen Han, Zafar Iqbal, Andrew M. Bailey, Christine L. Willis, Thomas J. Simpson and Russell J. Cox ()
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Risa Nofiani: University of Bristol
Kate Mattos-Shipley: University of Bristol
Karen E. Lebe: Leibniz Universität Hannover
Li-Chen Han: University of Bristol
Zafar Iqbal: University of Bristol
Andrew M. Bailey: University of Bristol
Christine L. Willis: University of Bristol
Thomas J. Simpson: University of Bristol
Russell J. Cox: University of Bristol

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract Strobilurins from fungi are the inspiration for the creation of the β-methoxyacrylate class of agricultural fungicides. However, molecular details of the biosynthesis of strobilurins have remained cryptic. Here we report the sequence of genomes of two fungi that produce strobilurins and show that each contains a biosynthetic gene cluster, which encodes a highly reducing polyketide synthase with very unusual C-terminal hydrolase and methyltransferase domains. Expression of stpks1 in Aspergillus oryzae leads to the production of prestrobilurin A when the fermentation is supplemented with a benzoyl coenzyme A (CoA) analogue. This enables the discovery of a previously unobserved route to benzoyl CoA. Reconstruction of the gene cluster in A. oryzae leads to the formation of prestrobilurin A, and addition of the gene str9 encoding an FAD-dependent oxygenase leads to the key oxidative rearrangement responsible for the creation of the β-methoxyacrylate toxophore. Finally, two methyltransferases are required to complete the synthesis.

Date: 2018
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DOI: 10.1038/s41467-018-06202-4

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