Ring synthetic chromosome V SCRaMbLE

Juan Wang, Ze-Xiong Xie, Yuan Ma, Xiang-Rong Chen, Yao-Qing Huang, Bo He, Jia, Bing-Zhi Li and Ying-Jin Yuan ()
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Juan Wang: Tianjin University
Ze-Xiong Xie: Tianjin University
Yuan Ma: Tianjin University
Xiang-Rong Chen: Tianjin University
Yao-Qing Huang: Tianjin University
Bo He: Tianjin University
Jia: Tianjin University
Bing-Zhi Li: Tianjin University
Ying-Jin Yuan: Tianjin University

Nature Communications, 2018, vol. 9, issue 1, 1-9

Abstract: Abstract Structural variations (SVs) exert important functional impacts on biological phenotypic diversity. Here we show a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic variations and improve the production of prodeoxyviolacein (PDV) by applying Synthetic Chromosome Recombination and Modification by LoxP-mediated Evolution (SCRaMbLE) in haploid yeast cells. The SCRaMbLE of ring_synV generates aneuploid yeast strains with increased PDV productivity, and we identify aneuploid chromosome I, III, VI, XII, XIII, and ring_synV. The neochromosome of SCRaMbLEd ring_synV generated more unbalanced forms of variations, including duplication, insertions, and balanced forms of translocations and inversions than its linear form. Furthermore, of the 29 novel SVs detected, 11 prompted the PDV biosynthesis; and the deletion of uncharacterized gene YER182W is related to the improvement of the PDV. Overall, the SCRaMbLEing ring_synV embraces the evolution of the genome by modifying the chromosome number, structure, and organization, identifying targets for phenotypic comprehension.

Date: 2018
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DOI: 10.1038/s41467-018-06216-y

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