Silencing of TGFβ signalling in microglia results in impaired homeostasis

Tanja Zöller, Artur Schneider, Christian Kleimeyer, Takahiro Masuda, Phani Sankar Potru, Dietmar Pfeifer, Thomas Blank, Marco Prinz and Björn Spittau ()
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Tanja Zöller: University of Freiburg
Artur Schneider: University of Freiburg
Christian Kleimeyer: University of Freiburg
Takahiro Masuda: University of Freiburg
Phani Sankar Potru: University of Freiburg
Dietmar Pfeifer: University of Freiburg
Thomas Blank: University of Freiburg
Marco Prinz: University of Freiburg
Björn Spittau: University of Freiburg

Nature Communications, 2018, vol. 9, issue 1, 1-13

Abstract: Abstract TGFβ1 has been implicated in regulating functional aspects of several distinct immune cell populations including central nervous system (CNS) resident microglia. Activation and priming of microglia have been demonstrated to contribute to the progression of neurodegenerative diseases and, thus, underlie stringent control by endogenous regulatory factors including TGFβ1. Here, we demonstrate that deletion of Tgfbr2 in adult postnatal microglia does neither result in impairment of the microglia-specific gene expression signatures, nor is microglial survival and maintenance affected. Tgfbr2-deficient microglia were characterised by distinct morphological changes and transcriptome analysis using RNAseq revealed that loss of TGFβ signalling results in upregulation of microglia activation and priming markers. Moreover, protein arrays demonstrated increased secretion of CXCL10 and CCL2 accompanied by activation of immune cell signalling as evidenced by increased phosphorylation of TAK1. Together, these data underline the importance of microglial TGFβ signalling to regulate microglia adaptive changes.

Date: 2018
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DOI: 10.1038/s41467-018-06224-y

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