Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations

Sonya A. MacParland (), Jeff C. Liu, Xue-Zhong Ma, Brendan T. Innes, Agata M. Bartczak, Blair K. Gage, Justin Manuel, Nicholas Khuu, Juan Echeverri, Ivan Linares, Rahul Gupta, Michael L. Cheng, Lewis Y. Liu, Damra Camat, Sai W. Chung, Rebecca K. Seliga, Zigong Shao, Elizabeth Lee, Shinichiro Ogawa, Mina Ogawa, Michael D. Wilson, Jason E. Fish, Markus Selzner, Anand Ghanekar, David Grant, Paul Greig, Gonzalo Sapisochin, Nazia Selzner, Neil Winegarden, Oyedele Adeyi, Gordon Keller, Gary D. Bader () and Ian D. McGilvray ()
Additional contact information
Sonya A. MacParland: Toronto General Hospital Research Institute
Jeff C. Liu: University of Toronto
Xue-Zhong Ma: Toronto General Hospital Research Institute
Brendan T. Innes: University of Toronto
Agata M. Bartczak: Toronto General Hospital Research Institute
Blair K. Gage: University Health Network
Justin Manuel: Toronto General Hospital Research Institute
Nicholas Khuu: University Health Network
Juan Echeverri: Toronto General Hospital Research Institute
Ivan Linares: Toronto General Hospital Research Institute
Rahul Gupta: Toronto General Hospital Research Institute
Michael L. Cheng: University of Toronto
Lewis Y. Liu: University of Toronto
Damra Camat: Toronto General Hospital Research Institute
Sai W. Chung: University of Toronto
Rebecca K. Seliga: Toronto General Hospital Research Institute
Zigong Shao: Toronto General Hospital Research Institute
Elizabeth Lee: Toronto General Hospital Research Institute
Shinichiro Ogawa: University Health Network
Mina Ogawa: University Health Network
Michael D. Wilson: University of Toronto
Jason E. Fish: University of Toronto
Markus Selzner: Toronto General Hospital Research Institute
Anand Ghanekar: Toronto General Hospital Research Institute
David Grant: Toronto General Hospital Research Institute
Paul Greig: Toronto General Hospital Research Institute
Gonzalo Sapisochin: Toronto General Hospital Research Institute
Nazia Selzner: Toronto General Hospital Research Institute
Neil Winegarden: University Health Network
Oyedele Adeyi: Toronto General Hospital Research Institute
Gordon Keller: University Health Network
Gary D. Bader: University of Toronto
Ian D. McGilvray: Toronto General Hospital Research Institute

Nature Communications, 2018, vol. 9, issue 1, 1-21

Abstract: Abstract The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal cells obtained from the fractionation of fresh hepatic tissue from five human livers. Using gene expression patterns, flow cytometry, and immunohistochemical examinations, we identify 20 discrete cell populations of hepatocytes, endothelial cells, cholangiocytes, hepatic stellate cells, B cells, conventional and non-conventional T cells, NK-like cells, and distinct intrahepatic monocyte/macrophage populations. Together, our study presents a comprehensive view of the human liver at single-cell resolution that outlines the characteristics of resident cells in the liver, and in particular provides a map of the human hepatic immune microenvironment.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06318-7

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DOI: 10.1038/s41467-018-06318-7

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