Regulatory control of DNA end resection by Sae2 phosphorylation

Elda Cannavo, Dominic Johnson, Sara N. Andres, Vera M. Kissling, Julia K. Reinert, Valerie Garcia, Dorothy A. Erie, Daniel Hess, Nicolas H. Thomä, Radoslav I. Enchev, Matthias Peter, R. Scott Williams, Matt J. Neale () and Petr Cejka ()
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Elda Cannavo: Università della Svizzera italiana (USI)
Dominic Johnson: University of Sussex
Sara N. Andres: US National Institutes of Health
Vera M. Kissling: Eidgenössische Technische Hochschule (ETH)
Julia K. Reinert: Friedrich Miescher Institute for Biomedical Research
Valerie Garcia: University of Sussex
Dorothy A. Erie: University of North Carolina
Daniel Hess: Friedrich Miescher Institute for Biomedical Research
Nicolas H. Thomä: Friedrich Miescher Institute for Biomedical Research
Radoslav I. Enchev: Eidgenössische Technische Hochschule (ETH)
Matthias Peter: Eidgenössische Technische Hochschule (ETH)
R. Scott Williams: US National Institutes of Health
Matt J. Neale: University of Sussex
Petr Cejka: Università della Svizzera italiana (USI)

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract DNA end resection plays a critical function in DNA double-strand break repair pathway choice. Resected DNA ends are refractory to end-joining mechanisms and are instead channeled to homology-directed repair. Using biochemical, genetic, and imaging methods, we show that phosphorylation of Saccharomyces cerevisiae Sae2 controls its capacity to promote the Mre11-Rad50-Xrs2 (MRX) nuclease to initiate resection of blocked DNA ends by at least two distinct mechanisms. First, DNA damage and cell cycle-dependent phosphorylation leads to Sae2 tetramerization. Second, and independently, phosphorylation of the conserved C-terminal domain of Sae2 is a prerequisite for its physical interaction with Rad50, which is also crucial to promote the MRX endonuclease. The lack of this interaction explains the phenotype of rad50S mutants defective in the processing of Spo11-bound DNA ends during meiotic recombination. Our results define how phosphorylation controls the initiation of DNA end resection and therefore the choice between the key DNA double-strand break repair mechanisms.

Date: 2018
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DOI: 10.1038/s41467-018-06417-5

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