RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis

Uwe Richter, Molly E. Evans, Wesley C. Clark, Paula Marttinen, Eric A. Shoubridge, Anu Suomalainen, Anna Wredenberg, Anna Wedell, Tao Pan () and Brendan J. Battersby ()
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Uwe Richter: University of Helsinki
Molly E. Evans: University of Chicago
Wesley C. Clark: University of Chicago
Paula Marttinen: University of Helsinki
Eric A. Shoubridge: McGill University
Anu Suomalainen: University of Helsinki
Anna Wredenberg: Karolinska Institutet
Anna Wedell: Karolinska University Hospital
Tao Pan: University of Chicago
Brendan J. Battersby: University of Helsinki

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract Post-transcriptional RNA modifications play a critical role in the pathogenesis of human mitochondrial disorders, but the mechanisms by which specific modifications affect mitochondrial protein synthesis remain poorly understood. Here we used a quantitative RNA sequencing approach to investigate, at nucleotide resolution, the stoichiometry and methyl modifications of the entire mitochondrial tRNA pool, and establish the relevance to human disease. We discovered that a N1-methyladenosine (m1A) modification is missing at position 58 in the mitochondrial tRNALys of patients with the mitochondrial DNA mutation m.8344 A > G associated with MERRF (myoclonus epilepsy, ragged-red fibers). By restoring the modification on the mitochondrial tRNALys, we demonstrated the importance of the m1A58 to translation elongation and the stability of selected nascent chains. Our data indicates regulation of post-transcriptional modifications on mitochondrial tRNAs is finely tuned for the control of mitochondrial gene expression. Collectively, our findings provide novel insight into the regulation of mitochondrial tRNAs and reveal greater complexity to the molecular pathogenesis of MERRF.

Date: 2018
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DOI: 10.1038/s41467-018-06471-z

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