Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast

Marc Larochelle, Marc-Antoine Robert, Jean-Nicolas Hébert, Xiaochuan Liu, Dominick Matteau, Sébastien Rodrigue, Bin Tian, Pierre-Étienne Jacques () and François Bachand ()
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Marc Larochelle: Université de Sherbrooke
Marc-Antoine Robert: Université de Sherbrooke
Jean-Nicolas Hébert: Université de Sherbrooke
Xiaochuan Liu: Rutgers New Jersey Medical School and Rutgers Cancer Institute of New Jersey
Dominick Matteau: Université de Sherbrooke
Sébastien Rodrigue: Université de Sherbrooke
Bin Tian: Rutgers New Jersey Medical School and Rutgers Cancer Institute of New Jersey
Pierre-Étienne Jacques: Université de Sherbrooke
François Bachand: Université de Sherbrooke

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Termination of RNA polymerase II (RNAPII) transcription is a fundamental step of gene expression that is critical for determining the borders between genes. In budding yeast, termination at protein-coding genes is initiated by the cleavage/polyadenylation machinery, whereas termination of most noncoding RNA (ncRNA) genes occurs via the Nrd1–Nab3–Sen1 (NNS) pathway. Here, we find that NNS-like transcription termination is not conserved in fission yeast. Rather, genome-wide analyses show global recruitment of mRNA 3′ end processing factors at the end of ncRNA genes, including snoRNAs and snRNAs, and that this recruitment coincides with high levels of Ser2 and Tyr1 phosphorylation on the RNAPII C-terminal domain. We also find that termination of mRNA and ncRNA transcription requires the conserved Ysh1/CPSF-73 and Dhp1/XRN2 nucleases, supporting widespread cleavage-dependent transcription termination in fission yeast. Our findings thus reveal that a common mode of transcription termination can produce functionally and structurally distinct types of polyadenylated and non-polyadenylated RNAs.

Date: 2018
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DOI: 10.1038/s41467-018-06546-x

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