Enhanced validation of antibodies for research applications

Fredrik Edfors, Andreas Hober, Klas Linderbäck, Gianluca Maddalo, Alireza Azimi, Åsa Sivertsson, Hanna Tegel, Sophia Hober, Cristina Al-Khalili Szigyarto, Linn Fagerberg, Kalle Feilitzen, Per Oksvold, Cecilia Lindskog, Björn Forsström and Mathias Uhlen ()
Additional contact information
Fredrik Edfors: KTH - Royal Institute of Technology
Andreas Hober: KTH - Royal Institute of Technology
Klas Linderbäck: KTH - Royal Institute of Technology
Gianluca Maddalo: KTH - Royal Institute of Technology
Alireza Azimi: Karolinska University Hospital
Åsa Sivertsson: KTH - Royal Institute of Technology
Hanna Tegel: KTH - Royal Institute of Technology
Sophia Hober: KTH - Royal Institute of Technology
Cristina Al-Khalili Szigyarto: KTH - Royal Institute of Technology
Linn Fagerberg: KTH - Royal Institute of Technology
Kalle Feilitzen: KTH - Royal Institute of Technology
Per Oksvold: KTH - Royal Institute of Technology
Cecilia Lindskog: Uppsala University
Björn Forsström: KTH - Royal Institute of Technology
Mathias Uhlen: KTH - Royal Institute of Technology

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation pillars were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that these principles can be used in a streamlined manner for enhanced validation of research antibodies in Western blot applications. More than 6,000 antibodies were validated with at least one of these strategies involving orthogonal methods, genetic knockdown, recombinant expression, independent antibodies, and capture mass spectrometry analysis. The results show a path forward for efforts to validate antibodies in an application-specific manner suitable for both providers and users.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06642-y

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DOI: 10.1038/s41467-018-06642-y

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