PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex

Stephanie Link, Ramona M. M. Spitzer, Maryam Sana, Mario Torrado, Moritz C. Völker-Albert, Eva C. Keilhauer, Thomas Burgold, Sebastian Pünzeler, Jason K. K. Low, Ida Lindström, Andrea Nist, Catherine Regnard, Thorsten Stiewe, Brian Hendrich, Axel Imhof, Matthias Mann, Joel P. Mackay, Marek Bartkuhn () and Sandra B. Hake ()
Additional contact information
Stephanie Link: BioMedical Center (BMC), Ludwig-Maximilians-University Munich
Ramona M. M. Spitzer: BioMedical Center (BMC), Ludwig-Maximilians-University Munich
Maryam Sana: School of Life and Environmental Sciences, University of Sydney
Mario Torrado: School of Life and Environmental Sciences, University of Sydney
Moritz C. Völker-Albert: BioMedical Center (BMC), Ludwig-Maximilians-University Munich
Eva C. Keilhauer: Max Planck Institute of Biochemistry
Thomas Burgold: University of Cambridge
Sebastian Pünzeler: BioMedical Center (BMC), Ludwig-Maximilians-University Munich
Jason K. K. Low: School of Life and Environmental Sciences, University of Sydney
Ida Lindström: School of Life and Environmental Sciences, University of Sydney
Andrea Nist: Genomics Core Facility, Philipps-University Marburg
Catherine Regnard: BioMedical Center (BMC), Ludwig-Maximilians-University Munich
Thorsten Stiewe: Genomics Core Facility, Philipps-University Marburg
Brian Hendrich: University of Cambridge
Axel Imhof: BioMedical Center (BMC), Ludwig-Maximilians-University Munich
Matthias Mann: Max Planck Institute of Biochemistry
Joel P. Mackay: School of Life and Environmental Sciences, University of Sydney
Marek Bartkuhn: Institute for Genetics, Justus-Liebig University Giessen
Sandra B. Hake: Institute for Genetics, Justus-Liebig University Giessen

Nature Communications, 2018, vol. 9, issue 1, 1-16

Abstract: Abstract Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified that PWWP2A tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial–facial development. Here, using in vitro assays, we show that distinct domains of PWWP2A mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly binds H3K36me3-containing gene bodies. Further, PWWP2A binds to an MTA1-specific subcomplex of the NuRD complex (M1HR), which consists solely of MTA1, HDAC1, and RBBP4/7, and excludes CHD, GATAD2 and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as a complex chromatin-binding protein that serves to direct the deacetylase complex M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels.

Date: 2018
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DOI: 10.1038/s41467-018-06665-5

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