DNA engineered micromotors powered by metal nanoparticles for motion based cellphone diagnostics

Mohamed Shehata Draz, Kamyar Mehrabi Kochehbyoki, Anish Vasan, Dheerendranath Battalapalli, Aparna Sreeram, Manoj Kumar Kanakasabapathy, Shantanu Kallakuri, Athe Tsibris, Daniel R. Kuritzkes and Hadi Shafiee ()
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Mohamed Shehata Draz: Harvard Medical School
Kamyar Mehrabi Kochehbyoki: Harvard Medical School
Anish Vasan: Harvard Medical School
Dheerendranath Battalapalli: Harvard Medical School
Aparna Sreeram: Harvard Medical School
Manoj Kumar Kanakasabapathy: Harvard Medical School
Shantanu Kallakuri: Harvard Medical School
Athe Tsibris: Harvard Medical School
Daniel R. Kuritzkes: Harvard Medical School
Hadi Shafiee: Harvard Medical School

Nature Communications, 2018, vol. 9, issue 1, 1-13

Abstract: Abstract HIV-1 infection is a major health threat in both developed and developing countries. The integration of mobile health approaches and bioengineered catalytic motors can allow the development of sensitive and portable technologies for HIV-1 management. Here, we report a platform that integrates cellphone-based optical sensing, loop-mediated isothermal DNA amplification and micromotor motion for molecular detection of HIV-1. The presence of HIV-1 RNA in a sample results in the formation of large-sized amplicons that reduce the motion of motors. The change in the motors motion can be accurately measured using a cellphone system as the biomarker for target nucleic acid detection. The presented platform allows the qualitative detection of HIV-1 (n = 54) with 99.1% specificity and 94.6% sensitivity at a clinically relevant threshold value of 1000 virus particles/ml. The cellphone system has the potential to enable the development of rapid and low-cost diagnostics for viruses and other infectious diseases.

Date: 2018
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DOI: 10.1038/s41467-018-06727-8

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