Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells

Joseph Jose Thottacherry, Anita Joanna Kosmalska, Amit Kumar, Amit Singh Vishen, Alberto Elosegui-Artola, Susav Pradhan, Sumit Sharma, Parvinder P. Singh, Marta C. Guadamillas, Natasha Chaudhary, Ram Vishwakarma, Xavier Trepat, Miguel A. Pozo, Robert G. Parton, Madan Rao, Pramod Pullarkat, Pere Roca-Cusachs and Satyajit Mayor ()
Additional contact information
Joseph Jose Thottacherry: Tata Institute of Fundamental Research (TIFR)
Anita Joanna Kosmalska: Institute for Bioengineering of Catalonia (IBEC)
Amit Kumar: Raman Research Institute
Amit Singh Vishen: Tata Institute of Fundamental Research (TIFR)
Alberto Elosegui-Artola: Institute for Bioengineering of Catalonia (IBEC)
Susav Pradhan: Raman Research Institute
Sumit Sharma: CSIR - Indian Institute of Integrative Medicine
Parvinder P. Singh: CSIR - Indian Institute of Integrative Medicine
Marta C. Guadamillas: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC)
Natasha Chaudhary: Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis
Ram Vishwakarma: CSIR - Indian Institute of Integrative Medicine
Xavier Trepat: Institute for Bioengineering of Catalonia (IBEC)
Miguel A. Pozo: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC)
Robert G. Parton: Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis
Madan Rao: Tata Institute of Fundamental Research (TIFR)
Pramod Pullarkat: Raman Research Institute
Pere Roca-Cusachs: Institute for Bioengineering of Catalonia (IBEC)
Satyajit Mayor: Tata Institute of Fundamental Research (TIFR)

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.

Date: 2018
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DOI: 10.1038/s41467-018-06738-5

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