CDC20B is required for deuterosome-mediated centriole production in multiciliated cells

Diego R. Revinski, Laure-Emmanuelle Zaragosi, Camille Boutin, Sandra Ruiz-Garcia, Marie Deprez, Virginie Thomé, Olivier Rosnet, Anne-Sophie Gay, Olivier Mercey, Agnès Paquet, Nicolas Pons, Gilles Ponzio, Brice Marcet (), Laurent Kodjabachian () and Pascal Barbry ()
Additional contact information
Diego R. Revinski: IBDM
Laure-Emmanuelle Zaragosi: Université Côte d’Azur, CNRS, IPMC
Camille Boutin: IBDM
Sandra Ruiz-Garcia: Université Côte d’Azur, CNRS, IPMC
Marie Deprez: Université Côte d’Azur, CNRS, IPMC
Virginie Thomé: IBDM
Olivier Rosnet: IBDM
Anne-Sophie Gay: Université Côte d’Azur, CNRS, IPMC
Olivier Mercey: Université Côte d’Azur, CNRS, IPMC
Agnès Paquet: Université Côte d’Azur, CNRS, IPMC
Nicolas Pons: Université Côte d’Azur, CNRS, IPMC
Gilles Ponzio: Université Côte d’Azur, CNRS, IPMC
Brice Marcet: Université Côte d’Azur, CNRS, IPMC
Laurent Kodjabachian: IBDM
Pascal Barbry: Université Côte d’Azur, CNRS, IPMC

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene, which hosts microRNA-449abc. We show that CDC20B protein associates to deuterosomes and is required for centriole release and subsequent cilia production in mouse and Xenopus MCCs. CDC20B interacts with PLK1, a kinase known to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescues centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules.

Date: 2018
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DOI: 10.1038/s41467-018-06768-z

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