Deacetylation of serine hydroxymethyl-transferase 2 by SIRT3 promotes colorectal carcinogenesis

Zhen Wei, Jinglue Song, Guanghui Wang, Ximao Cui, Jun Zheng, Yunlan Tang, Xinyuan Chen, Jixi Li, Long Cui (), Chen-Ying Liu () and Wei Yu ()
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Zhen Wei: Fudan University
Jinglue Song: Shanghai Jiao Tong University School of Medicine
Guanghui Wang: Shanghai Jiao Tong University School of Medicine
Ximao Cui: Shanghai Jiao Tong University School of Medicine
Jun Zheng: Fudan University
Yunlan Tang: Fudan University
Xinyuan Chen: Fudan University
Jixi Li: Fudan University
Long Cui: Shanghai Jiao Tong University School of Medicine
Chen-Ying Liu: Shanghai Jiao Tong University School of Medicine
Wei Yu: Fudan University

Nature Communications, 2018, vol. 9, issue 1, 1-16

Abstract: Abstract The conversion of serine and glycine that is accomplished by serine hydroxymethyltransferase 2 (SHMT2) in mitochondria is significantly upregulated in various cancers to support cancer cell proliferation. In this study, we observed that SHMT2 is acetylated at K95 in colorectal cancer (CRC) cells. SIRT3, the major deacetylase in mitochondria, is responsible for SHMT2 deacetylation. SHMT2-K95-Ac disrupts its functional tetramer structure and inhibits its enzymatic activity. SHMT2-K95-Ac also promotes its degradation via the K63-ubiquitin–lysosome pathway in a glucose-dependent manner. TRIM21 acts as an E3 ubiquitin ligase for SHMT2. SHMT2-K95-Ac decreases CRC cell proliferation and tumor growth in vivo through attenuation of serine consumption and reduction in NADPH levels. Finally, SHMT2-K95-Ac is significantly decreased in human CRC samples and is inversely associated with increased SIRT3 expression, which is correlated with poorer postoperative overall survival. Our study reveals the unknown mechanism of SHMT2 regulation by acetylation which is involved in colorectal carcinogenesis.

Date: 2018
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DOI: 10.1038/s41467-018-06812-y

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